The book, now in its third edition, continues to offer the basic concepts and principles of biochemical engineering. It covers the curriculum for a first-course in Biochemical Engineering at the undergraduate level of Chemical Engineering discipline and also caters to the requirements of B.Tech Biotechnology and BSc Biotechnology offered by various universities. The text first explains the basics of microbiology and biochemistry before moving on to explore the significance of enzymes, their properties, types, kinetics, industrial applications, production and formulation and the methods of their immobilization. It also deals with cell growth and its kinetic aspects and discusses various types of biological reactors with an emphasis on key engineering practices related to fermentation processes and products, bioreactor design and operation. It offers a complete description on downstream processing and control of micro-organisms. Besides, it also covers in the appendices some important topics such as process kinetics and reactor analysis, bioenergetics, and environmental microbiology to justify their relevance in biochemical engineering. It also offers a complete description with applications and configurations of membrane bioreactors (Chapter 7) and Presents a facelift of downstream processes in the topics, viz. disruption of cells supported with flow sheet, freeze drying, formulation, etc. along with a total revamping of the discussion on supercritical fluid extraction and induction of bio fouling (Chapter 9).