The book “Practical Guide Book on Genetics, Plant Breeding and Seed Technology” is a compilation of Practical Manuals of five different Under Graduate courses in the discipline of Genetics and Plant Breeding. It has been prepared as per revised syllabus approved and adopted by the Indian Council of Agricultural Research (ICAR), New Delhi for the Under Graduate courses in Agriculture. Every experiment in the book contains both theory and practical components. The basic information about each practical has been described in simple words for easy understanding of the students. Steps to be followed in conducting various experiments have also been given systematically. Examples have been given for easy understanding of the concepts. Exercises and activities to be performed by the students have been given with each practical so that the students can test their knowledge about each topic. List of reference books and sources of online information have also been listed for future consultation by the students. The practical experiment covered in the book includes description of the compound microscope, cell structure and its organelles, process of cell division- mitosis and meiosis, principle and exercise on Mendelian inheritance, Chi-square (χ2) test, 2-point and 3-point test cross, linkage map construction, description about model organisms (Drosophila, Neurospora, Arabidopsis, etc), structure of DNA and RNA, flower biology and emasculation and pollination techniques in 28 rabi and kharif crops, seed production technologies of 17 field and horticultural crops, testing of seeds for germination, genetic purity, pollination behaviour of self- and cross-pollinated crops, understanding various male sterility system, estimation of mean, variance, heritability, standard deviation (SD), and coefficient of variation (CV), lay out of field experiments in RBD, ANOVA and test of significance, prediction of performance of double-cross hybrids, principles of population handing through