ISBN 9788180521430,Manual of Microbiology Tools & Techniques

Manual of Microbiology Tools & Techniques



Ane Books Pvt. Ltd

Publication Year 2011

ISBN 9788180521430

ISBN-10 8180521435

Paper Back

Number of Pages 424 Pages
Language (English)


The basic concepts of microbial tools and techniques were assembled in an organized and informative manner in the first edition of "Manual of Microbiology". Although an attempt was made to include most of the salient information in this book, but later it was felt that the book was still lacking in some areas. So it was decided to bring out the second edition of the book to make it more informative and to rectify the mistakes.

Several additions have been made in the second edition to make it more informative. Additions to the first chapter of revised edition include a concise description of history of Microbiology, structure and reproduction of bacteria, detailed list of differences between Prokaryotes & Eukaryotes, a brief history as well as an overview of Bergey's Manual of Systematic Bacteriology and salient features of some important bacterial groups. Improvements have been made in the second and third chapters also which offer an insight into the various tools and techniques of microbiology.

These additions include description of some new generation microscopes, difference between boiling and autoclaving, substances which should not be autoclaved, testing of autoclave, preparation of specimens for EM viewing, inoculation techniques, preparation, incubation and disposal of used plates, replica plating method etc. All information regarding culture media and methods of isolation, purification and culture of bacteria along with some additions have now been organized together in chapter 4.

A detailed description of different methods used to isolate and identify bacteria according to the Bergey`s manual and to maintain pure cultures of them have been included in Chapters 5 and 6. Some new exercises have been added to Chapters 7 to 15 which already contain several simple experiments that help in understanding the physical and chemical nature of microbes as well as important metabolic processes such as growth, effect of environmental factors on growth, bacterial genetics, control of microbes, water, soil, food and milk microbiology. Chapters 16, 17 and 18 of this book aim to improve the understanding of microbes other than bacteria, similarly Chapters 19 and 20 suggest methods to isolate and study plant and human pathogens especially bacteria. All these experiments can be performed easily in practical classes at graduate as well as postgraduate level and will also be of great use for teachers and students involved in teaching and research.