Biotechnology is a broad subject. It covers all the basic subject of tissue culture, transformation technology, molecular marker technology, genetics and genomics. Biotechnology courses have been included in the basic course curriculum of school and university students. Therefore, students have to pass through all the biotechnological related subject during under graduation and post graduation. This book has been offered with the aim to provide a large number of questions in all major portions of cell structure and function, bio molecule and metabolism, enzymology, molecular biology, gene cloning, molecular markers and genomics. This “Objective Book of Agriculture Biotechnology will helpful for the students preparing for various competitive examinations such as NET/JRF/SRF/ARS where multiple type questions are asked.