This textbook has been designed to meet the needs of B.Sc. Second Semester students of Chemistry as per Common Minimum Syllabus prescribed for all Uttar Pradesh State Universities and Colleges under the recommended National Education Policy 2020. Maintaining the traditional approach to the subject, this textbook comprehensively covers two papers, namely, Bioorganic and Medicinal Chemistry and Biochemical Analysis. Important theoretical topics such as chemistry of carbohydrates, proteins & nucleic acids, laws of crystallography, introduction & classification of monomers, oligomers, polymers are aptly discussed to give an overview of Bioorganic and Medicinal Chemistry. Practical part covering Biochemical Analysis has been presented systematically to help students achieve solid conceptual understanding and learn experimental procedures.